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CHAPTER 32: Biotechnology: Principles and Processes

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Question 1 of 15

1. Question
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
- The smaller the fragment size, the farther it moves.
- Positively charged fragments move to farther end.
- Negatively charged fragments do not move.
- The larger the fragment size, the farther it moves.
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Question 2 of 15

2. Question
Stirredtank bioreactors have been designed for
- Purification of product
- Addition of preservatives to the product
- Availability of oxygen throughout the process
- Ensuring anaerobic conditions in the culture vessel
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Question 3 of 15

3. Question
- 4
- 2
- 1
- 3
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Question 4 of 15

4. Question
Biolistics (genegun) is suitable for
- Disarming pathogen vectors
- Transformation of plant cells
- Constructing recombinant DNA by joining with vectors
- DNA fingerprinting.
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Question 5 of 15

5. Question
Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it?

5¢_____ GAATTC¢ _____ 3¢

3¢_____ CTTAAG _____ 5¢
- Replication completed
- Deletion mutation
- Start codon at the 5¢ end
- Palindromic sequence of base pairs
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Question 6 of 15

6. Question
- c
- d
- b
- a
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Question 7 of 15

7. Question
Which of the following statement is not correct about cloning vector?
- ‘Ori’ is a sequence responsible for controlling the copy number of the linked DNA.
- Selectable marker selectively permitting the growth of the non-transformants.
- To link the alien DNA, the vector needs to have single recognition site for the commonly used restriction enzymes
- The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes
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Question 8 of 15

8. Question
After 4 PCR cycles how many DNA molecules are formed from one DNA template molecule ?
- 4
- 32
- 16
- 8
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Question 9 of 15

9. Question
The DNA fragments separated on an agarose gel can be visualised after staining with
- Acetocarmine
- Aniline blue
- Ethidium bromide
- Bromophenol blue
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Question 10 of 15

10. Question
A gene whose expression helps to identify transformed cell is known as
- Vector
- Plasmid
- Structural gene
- Selectable marker.
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Question 11 of 15

11. Question
- 4
- 3
- 2
- 1
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Question 12 of 15

12. Question
The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of
- Insertional inactivate of alphaga-lactosidase in non-recombinant bacteria.
- Insertional inactivation of alphagalactosidase in recombinant bacteria.
- Inactivation of glycosidase enzyme in recombinant bacteria.
- Non-recombinant bacteria containing betagalactosidase
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Question 13 of 15

13. Question
Identify the correct match for the given figure
- Electrophoresis Differential migration of DNA fragments
- Column Separation of chlorophyll chromatography pigments
- Gene cloning Technique of obtaining identical copies of a particular DNA segment or a gene
- Microinjection Technique of introducing foreign genes into a host cell
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Question 14 of 15

14. Question
Which of these best shows that plant cells are totipotent ?
- A shoot culture for the purpose of micropropagation
- Flower meristem culture for the purpose of somatic embryos
- Protoplasmic culture for the purpose of genetic engineering of plants
- Leaf, stem and root culture for the purpose of cell suspension cultures
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Question 15 of 15

15. Question
Which one of the following bacteria has found extensive use in genetic engineering work in plants?
- Clostridium septicum
- Xanthomonas citri
- Bacillus coagulens
- Agrobacterium tumefaciens
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